Dicle Üniversitesi Fen Bilimleri Enstitüsü Dergisi

Yazarlar: Cemil DEMİR, Songül ÇETİK YILDIZ

Konular:Biyoloji

Anahtar Kelimeler:Staphylococcus,Slime,PCR,Kongo red,Enfeksiyon

Özet: In this study, Staphylococus aureus strain was isolated in 125 of the samples of 180 patients’wounds who were hospitalized in Mardin State Hospital Orthopedic Service and implanted, while Staphylococus epidermitis strain was isolated in 62 of them. In these isolates, it was aimed to determine the presence of ica A and ica D genes responsible for slime production by Multiplex PCR, to determine slime production by phenotypic methods such as Congo red agar (CRA), standard test tube (ST) and microplate (MP) and to determine the role of the slime (biofilm) layer in the resistance mechanism by looking at the antibiotic resistance of the strains with disc diffusion agar. While the CRA, MP and ST tests of the staphylococcus strains examined were found to be respectively 62.4%, 57.7% and 52.9% positive, the ica A and ica D gene positivity was 65.6% (82/125). It was determined that all staphylococci examined were sensitive to teicoplanin and vancomycin antibiotics. While antibiotic resistance rates were higher in slime positive strains than slime negative strains, only penicillin, ampicillin and erythromycin resistance rates were found statistically significant. As a result, resistance to antibiotics was found to be significant in strains positive with regard to ica A and ica D genes.

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