Black Sea Journal of Agriculture

Yazarlar: Leyla BENER, Rabia YAŞAR, Mehmet KURAN

Konular:Mühendislik, Ziraat

Anahtar Kelimeler:Embryo,Culture medium,Heat shock,Proteins,Apoptosis

Özet: For a long period of time significant progress attained in in vitro embryo production. Despite desired levels has not been reached  the main reason are inability to imitate in vivo conditions of in vitro culture medium precisely and embryo be exposed to stress factors such as environmental conditions, temperature and biological components as a reaction produce stress proteins. Stress proteins which are increased in the organism during culture medium components, serums (FCS, ECS), UV rays, toxin, arsenic, pH, ischemia, oxidative phosphorylation, cold and hot conditions and during infections and vary in size between 100 to 8 kDA. HSP70 has two main domains, compact amino terminal site and low ATPase activity which been highly conserved and it regulate heat shock activity. During in vitro embryo production, the effect of environmental stress and embryo development processes in relation with the roll of HSP70 is very important. Presence of serum in the culture medium thought that it decreased the embryo development rate. In this review, we attempted to explain heat shock proteins and their varieties produced in vitro by oocyte maturation and embryo production. Furthermore, the possible effects of these proteins on in vitro embryo production activity have been discussed.

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