Turkish Journal of Veterinary and Animal Sciences

Yazarlar: Sema BİRLER, Serhat PABUÇÇUOĞLU, Serhat ALKAN, Mithat EVECEN, İ. Kamuran İLERİ

Konular:-

Anahtar Kelimeler:Turk. J. Vet. Anim. Sci. 1998,22: 1-16. Full text: pdf Other articles published in the same issue: Turk. J. Vet. Anim. Sci.,Vol.22,Iss.1.

Özet: Primer oocytes (n=520) collected from ovaries of slaughtered cows were divided into 3 groups, and matured at 39ºC with 95-100% humidity and under a gas mixture of 5% O 2 , 5% CO 2 and 90% N 2 for 22, 24 and 26 hours. Modified Parker's Medium (MPM) supplemented with FSH and 20% ECS was used as maturation medium. At the end of these periods, oocytes (22 h.=122, 24 h.=143 and 26 h.=255) were taken into Tyrode-lactate medium supplemented with BSA, heparin and PHE, and frozen-thawed bull semen was add after swim-up procedure (10µ[email protected] spermatozoa/30-35 oocytes). Oocytes matured in 3 groups were kept with spermatozoa for 14, 16, 18, 20, 22 and 24 hours. At the end of these time periods all oocytes were fixed, stained and examined by phase-contrast microscope. In total 42 (72.4%), 38 (69.1%) and 100 (84.7%) oocytes relating to the maturation groups, male and female pronuclei were synchronised. Difference between the 26 h. group and the other groups was highly significant (c 2 , p

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