Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

Isolation and Preparation of Monoclonal Antibody to Ovine Immunoglobulin M

Yazarlar: Hüseyin YILMAZ, Aysan TANTAŞ, Atilla ILGAZ

Cilt 23 , Sayı 2 , 1999 , Sayfalar 135 - 140

Konular:-

Anahtar Kelimeler:IgM,Isolation,Monoclonal,Antibody.

Özet: Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.


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BibTex
KOPYALA
@article{1999, title={Isolation and Preparation of Monoclonal Antibody to Ovine Immunoglobulin M}, volume={23}, number={2}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Hüseyin YILMAZ,Aysan TANTAŞ,Atilla ILGAZ}, year={1999}, pages={135–140} }
APA
KOPYALA
Hüseyin YILMAZ,Aysan TANTAŞ,Atilla ILGAZ. (1999). Isolation and Preparation of Monoclonal Antibody to Ovine Immunoglobulin M (Vol. 23, pp. 135–140). Vol. 23, pp. 135–140. Turkish Journal of Veterinary and Animal Sciences.
MLA
KOPYALA
Hüseyin YILMAZ,Aysan TANTAŞ,Atilla ILGAZ. Isolation and Preparation of Monoclonal Antibody to Ovine Immunoglobulin M. no. 2, Turkish Journal of Veterinary and Animal Sciences, 1999, pp. 135–40.