Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears

Yazarlar: Zati VATANSEVER, Serpil NALBANTOĞLU

Cilt 26 , Sayı 6 , 2002 , Sayfalar 1465 - 1469

Konular:-

Anahtar Kelimeler:Theileria annulata,PCR,Nested PCR,IFAT,Epidemiology

Özet: The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in four localities of Polatlı district, Ankara, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3%, 44.9% and 61.2% for microscopy, IFAT and nested PCR, respectively. It was shown that nested PCR is more sensitive and specific when compared to microscopical examination and serological findings. It was also shown that nested PCR is more sensitive than single step PCR. This study, which is the first work utilising nested PCR in the detection of T. annulata in Turkey, showed that nested PCR is more sensitive and specific when compared to microscopy and IFAT and can be used in further epidemiological studies.

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@article{2002, title={Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears}, volume={26}, number={6}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Zati VATANSEVER,Serpil NALBANTOĞLU}, year={2002}, pages={1465–1469} }
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Zati VATANSEVER,Serpil NALBANTOĞLU. (2002). Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears (Vol. 26, pp. 1465–1469). Vol. 26, pp. 1465–1469. Turkish Journal of Veterinary and Animal Sciences.
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Zati VATANSEVER,Serpil NALBANTOĞLU. Detection of Cattle Infected with Theileria Annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears. no. 6, Turkish Journal of Veterinary and Animal Sciences, 2002, pp. 1465–69.
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