Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

Detection of Infectious Bronchitis Virus in Tracheal Organ Cultures Using the Immunoperoxidase Technique

Yazarlar: Ayşin ŞEN

Cilt 26 , Sayı 6 , 2002 , Sayfalar 1381 - 1388

Konular:-

Anahtar Kelimeler:Infectious bronchitis virus,Tracheal organ cultures,Immunoperoxidase

Özet: The applicability of the immunoperoxidase (IP) technique in unfixed-fresh tracheal organ cultures (TOCs) was investigated for rapid detection of infectious bronchitis virus (IBV). For this purpose, TOCs prepared from one-day-old SPF chicks were inoculated with IBV M41 strain in serial dilutions. IBV antigen detected by IP technique in TOCs 18 h postinoculation. IP staining was observed before siliostasis in all dilutions. The IP technique was also applied to detect IBV antigen in formalin-fixed, paraffin-embedded sections of TOCs inoculated with IBV M41 strain. Viral antigen was also detected in these sections. Histopathological examinations of IBV M41 infected tracheal rings showed loss of cilia, flattening of lamina epithelialis, and rounding and desquamation of epithelial cells.

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@article{2002, title={Detection of Infectious Bronchitis Virus in Tracheal Organ Cultures Using the Immunoperoxidase Technique}, volume={26}, number={6}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Ayşin ŞEN}, year={2002}, pages={1381–1388} }
APA
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Ayşin ŞEN. (2002). Detection of Infectious Bronchitis Virus in Tracheal Organ Cultures Using the Immunoperoxidase Technique (Vol. 26, pp. 1381–1388). Vol. 26, pp. 1381–1388. Turkish Journal of Veterinary and Animal Sciences.
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Ayşin ŞEN. Detection of Infectious Bronchitis Virus in Tracheal Organ Cultures Using the Immunoperoxidase Technique. no. 6, Turkish Journal of Veterinary and Animal Sciences, 2002, pp. 1381–88.
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