Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

The Use of Recombinant Hemagglutinine Protein of Rinderpest Virus in Enzyme Immunoassay

Yazarlar: Hakan BULUT, Yusuf BOLAT

Cilt 27 , Sayı 1 , 2003 , Sayfalar 249 - 252

Konular:-

Anahtar Kelimeler:Rinderpest virus,Recombinant hemagglutinine protein,Enzyme immunoassay

Özet: In this study, Rinderpest virus (RPV) recombinant hemagglutinine protein (rH) fused with protein A region of Staphylococcus aureus was expressed in Escherichia coli and purified by IgG affinity chromatography. rH protein was also used to establish enzyme immunoassay. Therefore, to prevent IgG binding to the protein A the wells coated with the rH proteins were blocked by human serum. Afterwards, RPV antigens were added to the wells to evaluate this assay. To this end, serum from mice immunized with RPV was used for EIA. Our data indicated that the rH protein fused with protein A of Staphylococcus aureus can be reliably used for enzyme immunoassays.

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@article{2003, title={The Use of Recombinant Hemagglutinine Protein of Rinderpest Virus in Enzyme Immunoassay}, volume={27}, number={1}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Hakan BULUT,Yusuf BOLAT}, year={2003}, pages={249–252} }
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Hakan BULUT,Yusuf BOLAT. (2003). The Use of Recombinant Hemagglutinine Protein of Rinderpest Virus in Enzyme Immunoassay (Vol. 27, pp. 249–252). Vol. 27, pp. 249–252. Turkish Journal of Veterinary and Animal Sciences.
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Hakan BULUT,Yusuf BOLAT. The Use of Recombinant Hemagglutinine Protein of Rinderpest Virus in Enzyme Immunoassay. no. 1, Turkish Journal of Veterinary and Animal Sciences, 2003, pp. 249–52.
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