Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

Development of an Enzyme Immunoassay for the Determination of Testosterone

Yazarlar: Bülent GÜVEN

Cilt 27 , Sayı 1 , 2003 , Sayfalar 45 - 50

Konular:-

Anahtar Kelimeler:Testosterone,Enzyme immunoassay

Özet: In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to be


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BibTex
KOPYALA
@article{2003, title={Development of an Enzyme Immunoassay for the Determination of Testosterone}, volume={27}, number={1}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Bülent GÜVEN}, year={2003}, pages={45–50} }
APA
KOPYALA
Bülent GÜVEN. (2003). Development of an Enzyme Immunoassay for the Determination of Testosterone (Vol. 27, pp. 45–50). Vol. 27, pp. 45–50. Turkish Journal of Veterinary and Animal Sciences.
MLA
KOPYALA
Bülent GÜVEN. Development of an Enzyme Immunoassay for the Determination of Testosterone. no. 1, Turkish Journal of Veterinary and Animal Sciences, 2003, pp. 45–50.