Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels

Yazarlar: Ergün Ö. GÖKSOY, Şükrü KIRKAN, Osman KAYA

Cilt 30 , Sayı 2 , 2006 , Sayfalar 229 - 234

Konular:-

Anahtar Kelimeler:Listeria monocytogenes,Stuffed mussel,Conventional methods,PCR

Özet: The aim of this study was to evaluate the role of stuffed mussel as a source of Listeria monocytogenes. Polymerase chain reaction is a rapid procedure with both sensitivity and specificity for quick detection and identification of L. monocytogenes. A total of 50 mussel samples were investigated for L. monocytogenes. L. monocytogenes was not identified by the conventional method. However, PCR amplification products demonstrated that 5 out of 50 samples showed positive reactions with L. monocytogenes. The PCR positive samples showed specific amplification at approximately 343 bp for L. monocytogenes.

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@article{2006, title={Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels}, volume={30}, number={2}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Ergün Ö. GÖKSOY,Şükrü KIRKAN,Osman KAYA}, year={2006}, pages={229–234} }
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Ergün Ö. GÖKSOY,Şükrü KIRKAN,Osman KAYA. (2006). Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria monocytogenes in Stuffed Mussels (Vol. 30, pp. 229–234). Vol. 30, pp. 229–234. Turkish Journal of Veterinary and Animal Sciences.
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Ergün Ö. GÖKSOY,Şükrü KIRKAN,Osman KAYA. Comparison of Polymerase Chain Reaction and Conventional Methods for the Diagnosis of Listeria Monocytogenes in Stuffed Mussels. no. 2, Turkish Journal of Veterinary and Animal Sciences, 2006, pp. 229–34.
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