Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

Detection of the eaeA Gene in Escherichia coli from Chickens by Polymerase Chain Reaction

Yazarlar: Ayşe KILIÇ, Hasan Basri ERTAŞ, Adile MUZ, Gökben ÖZBEY

Cilt 31 , Sayı 4 , 2007 , Sayfalar 215 - 218

Konular:-

Anahtar Kelimeler:Escherichia coli,PCR,Chicken,EaeA gene

Özet: The aim of this study was to isolate Escherichia coli from chickens and to determine the presence of the eaeA gene, a virulence factor detected in E. coli, in the isolates by polymerase chain reaction (PCR). Different chicken organs were inoculated onto blood agar and biochemical tests were performed on the suspicious isolates. E. coli was isolated from 48% (48/100) of the samples. DNA was extracted from these isolates and was amplified by PCR, using a pair of primers derived from the eaeA (virulence) gene. In the agarose gel examination of PCR products, 48% (48/100) of the isolates were determined to have this virulence factor.

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@article{2007, title={Detection of the eaeA Gene in Escherichia coli from Chickens by Polymerase Chain Reaction}, volume={31}, number={4}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Ayşe KILIÇ,Hasan Basri ERTAŞ,Adile MUZ,Gökben ÖZBEY}, year={2007}, pages={215–218} }
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Ayşe KILIÇ,Hasan Basri ERTAŞ,Adile MUZ,Gökben ÖZBEY. (2007). Detection of the eaeA Gene in Escherichia coli from Chickens by Polymerase Chain Reaction (Vol. 31, pp. 215–218). Vol. 31, pp. 215–218. Turkish Journal of Veterinary and Animal Sciences.
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Ayşe KILIÇ,Hasan Basri ERTAŞ,Adile MUZ,Gökben ÖZBEY. Detection of the EaeA Gene in Escherichia Coli from Chickens by Polymerase Chain Reaction. no. 4, Turkish Journal of Veterinary and Animal Sciences, 2007, pp. 215–18.
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