Turkish Journal of Veterinary and Animal Sciences

Yazarlar: Hui-fang LI, Wen-qi ZHU, Kuan-wei CHEN, Xu WU, Qing-ping TANG, Yu-shi GAO

Konular:-

Anahtar Kelimeler:Chicken,IGF-1,GHR,Reproduction,Single nucleotide polymorphism (SNP)

Özet: Alleles of physiological candidate genes for reproductive traits, insulin-like growth factor-1 (IGF-1), and growth hormone receptor (GHR) were assessed to determine their associations with total egg production (NE), average days of continual egg-laying (ADCE), and number of double yolk eggs (DYE) in Wenchang chickens (Chinese indigenous breed). PCR-RFLP was used for genotype identification. The frequency of restriction enzyme C1/C2 alleles in the population was 0.53 (C1) and 0.47 (C2) for IGF-1. For GHR-intron 2 it was 0.06 and 0.94 for (A1) and (A2), respectively, while for GHR-intron 5 it was 0.20 (B1) and 0.80 (B2). Four significant associations were found (P < 0.05): between IGF-1 polymorphism, and NE (300 d), NE (400 d), and ADCE, and between GHR polymorphism and DYE. Two significant effects were observed: for IGF-1 and NE (300 d), and for GHR intron 2 and DYE. The current research supports the effects of GHR and IGF-1 genes on the reproductive traits of chickens.

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