Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia

Yazarlar: Mutlu KÜÇÜK, Mehmet KAYA, Nurcan ORHAN, Oğuzhan EKİZOĞLU, Erol ARSLAN, Nadir ARICAN, R.candan GÜRSES, İmdat ELMAS, Rivaze KALAYCI, Bülent AHISHALI

Cilt 36 , Sayı 3 , 2012 , Sayfalar 311 - 318

Konular:-

DOI:10.3906/sag-1203-43

Anahtar Kelimeler:Key words: Pregnancy,Hypertension,Sodium fluorescein,Aquaporin-4,Occluding

Özet: The pathophysiological mechanisms underlying blood-brain barrier (BBB) disruption in preeclampsia/eclampsia have not been elucidated. This study investigated the effect of pentylenetetrazole (PTZ)-induced seizures on the functional and structural properties of the BBB during N (omega)-nitro-L-arginine methyl ester (L-NAME)-induced hypertension and proteinuria in pregnant rats. Animals were treated with L-NAME for 10 days, beginning on day 10 of pregnancy. The BBB permeability was determined by measurements of sodium fluorescein (NaFlu) extravasation into the brain. Occludin and aquaporin (AQP)-4 immunoreactivities were evaluated in brain sections. Severe proteinuria and significantly increased arterial blood pressure were observed following L-NAME treatment. PTZ-induced seizures in pregnant rats treated with L-NAME increased NaFlu levels in all of the brain regions analyzed (P < 0.01). A significant increase in the extravasation of NaFlu was also observed in the diencephalon of intact pregnant rats treated with PTZ. PTZ-induced seizures in both L-NAME-treated and untreated pregnant rats significantly decreased occludin immunoreactivity in the hippocampal capillaries. L-NAME administration significantly increased AQP-4 immunoreactivity in the astrocytic endfeet surrounding the parietal cortex microvessels of PTZ-treated and untreated pregnant rats. These findings suggest that PTZ-induced seizures lead to a severe disruption of the BBB in preeclampsia and that the paracellular pathway may play an important role in increased BBB permeability in this context.


ATIFLAR
Atıf Yapan Eserler
Henüz Atıf Yapılmamıştır

KAYNAK GÖSTER
BibTex
KOPYALA
@article{2012, title={The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia}, volume={36}, number={3}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Mutlu KÜÇÜK,Mehmet KAYA,Nurcan ORHAN,Oğuzhan EKİZOĞLU,Erol ARSLAN,Nadir ARICAN,R.candan GÜRSES,İmdat ELMAS,Rivaze KALAYCI,Bülent AHISHALI}, year={2012}, pages={311–318} }
APA
KOPYALA
Mutlu KÜÇÜK,Mehmet KAYA,Nurcan ORHAN,Oğuzhan EKİZOĞLU,Erol ARSLAN,Nadir ARICAN,R.candan GÜRSES,İmdat ELMAS,Rivaze KALAYCI,Bülent AHISHALI. (2012). The effect of pentylenetetrazole-induced seizures on blood-brain barrier integrity in a rat model of preeclampsia (Vol. 36, pp. 311–318). Vol. 36, pp. 311–318. Turkish Journal of Veterinary and Animal Sciences.
MLA
KOPYALA
Mutlu KÜÇÜK,Mehmet KAYA,Nurcan ORHAN,Oğuzhan EKİZOĞLU,Erol ARSLAN,Nadir ARICAN,R.candan GÜRSES,İmdat ELMAS,Rivaze KALAYCI,Bülent AHISHALI. The Effect of Pentylenetetrazole-Induced Seizures on Blood-Brain Barrier Integrity in a Rat Model of Preeclampsia. no. 3, Turkish Journal of Veterinary and Animal Sciences, 2012, pp. 311–18.