Turkish Journal of Veterinary and Animal Sciences

Turkish Journal of Veterinary and Animal Sciences

Glycohistochemistry of the lateral tympanic membrane in the syrinx of the Denizli cock

Yazarlar: Nazan KESKİN, Pınar İLİ

Cilt 37 , Sayı 4 , 2013 , Sayfalar 414 - 418

Konular:-

DOI:10.3906/tar-1203-3

Anahtar Kelimeler:Key words: Denizli cock,Syrinx,Tympanic membrane,Glycostructures

Özet: The Denizli cock is a special race that has harmonious long crowing. In this study, the syrinx of the Denizli cock was studied by using glycohistochemical analyses. Paraffin sections were stained with Alcian blue (AB) at pH 1 and pH 2.5 for demonstration of sulfated and carboxylated carbohydrates, respectively. At AB-pH 1, the lateral tympanic membrane (LTM) and also the medial tympanic membrane (MTM) exhibited a weak reactivity, whereas they were stained intensely at AB-pH 2.5. The frozen sections were incubated with 5 digoxigenin-labeled lectins for lectin histochemistry: peanut agglutinin, Maackia amurensis leucoagglutinin, Galanthus nivalis agglutinin, Datura stramonium agglutinin, and Sambucus nigra agglutinin. In LTM, positive stainings were observed for the 5 lectins. These results suggest that LTM exhibits an intense reactivity with AB-pH 2.5, indicating carboxylated carbohydrates, and it also has terminal saccharide moieties consisting of b(1->3) linked galactose, a(2->3) linked sialic acid, mannose, b (1->4) linked galactose, and a (2->6) linked sialic acid.


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BibTex
KOPYALA
@article{2013, title={Glycohistochemistry of the lateral tympanic membrane in the syrinx of the Denizli cock}, volume={37}, number={4}, publisher={Turkish Journal of Veterinary and Animal Sciences}, author={Nazan KESKİN,Pınar İLİ}, year={2013}, pages={414–418} }
APA
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Nazan KESKİN,Pınar İLİ. (2013). Glycohistochemistry of the lateral tympanic membrane in the syrinx of the Denizli cock (Vol. 37, pp. 414–418). Vol. 37, pp. 414–418. Turkish Journal of Veterinary and Animal Sciences.
MLA
KOPYALA
Nazan KESKİN,Pınar İLİ. Glycohistochemistry of the Lateral Tympanic Membrane in the Syrinx of the Denizli Cock. no. 4, Turkish Journal of Veterinary and Animal Sciences, 2013, pp. 414–18.