Eurasian Journal of Biological and Chemical Sciences

Yazarlar: İlhami TUZUN, Merve KAVASBASIOGLU, Yaşar ALUÇ

Konular:Biyoloji

Anahtar Kelimeler:Mikroalg,Chlorella vulgaris,Amino asit,Protein,HPLC analizi

Özet: The microalgae species in this study, Chlorella vulgaris, isolated from Kapulukaya Reservoir (Kırıkkale), were subjected to four different growth media, being (i) Tris-Acetat-Phosphate (TAP), (ii) Tris-Acetat-Phosphate enriched with N (N-TAP), (iii) Kırıkkale University Campus Lake Water (LW) and (iv) Waste Water from Mechanical and Chemical Industry Company (MKEK) in order to compare its growth by monitoring the parameters, cell count (CC), Optical density (OD) and Chlorophyll-a (Chl-a). Of the experimental trials, all of which reached the stationary phase on day 15, the highest biomass was detected in the LW medium. Following the end of the experiments, amounts of total protein measured on the harvested biomass by using Lowry method were found to be 2,17 mg/BSA (N-TAP), 1,96 mg/BSA (TAP), 0,87 mg/BSA (MKEK) and 0,69 mg/BSA (LW). Results from anion measurements in the media suggested the idea that phosphate limitations occurred during growth but being onset earlier in the MKEK and LW media than that in the TAP and TAP-N media. The greater protein content found in TAP and TAP-N media corresponded with similarly higher values obtained from Fourier Transform Infrared Spectroscopy (FTIR) analysis. The amount of arginine was profoundly higher amongst amino acids. Both essential and non-essential amino acids were five-fold higher in N-TAP and TAP media compared to wastewater (MKEK) and LW. This suggested a general conclusion that the used natural waters were not compatible enough with the controlled media due mainly to that in natural waters phosphorus limited the growth despite efficient N levels.

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