Eurasian Journal of Biological and Chemical Sciences

Yazarlar: Beyza Sultan AYDIN, İ̇brahim BULDUK

Konular:Mühendislik, Kimya

Anahtar Kelimeler:Dopamin HCl,HPLC,Method,Validation

Özet: Dopamine is a neurotransmitter that acts in the nervous system as a chemical messenger, which helps specific neurons to interact with each other. A simple, precise, accurate HPLC method has been developed and validated for determination of Dopamine HCl (DOPHCl) in pharmaceutical formulations. Separation was performed on a C18 column (250 x 4.6 mm, 5 μm) with 50 mM potassium dihydrogen phosphate (pH 2.3) as a mobile phase. Mobile phase flow rate was maintained constant at 1 mL min-1. High sensitivity has been achieved with UV detection at 280 nm. Parameters such as interaction of the active pharmaceutical ingredient and excipients, linearity and repeatability were investigated during analytical method development. Retention time was 6.195 min for DOPHCl. The method has been validated for DOPHCl within the range of 20-100 μg mL-1 (r2=0.9998). This method has shown strong reproducibility and recovery within the determined range. This method can be used successfully in pharmaceutical formulations for routine analysis.

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