Eurasian Journal of Biological and Chemical Sciences

Yazarlar: Elif AKSÖZ, Bükay YENİCE GÜRSU, Büşra ASLAN, Betül YILMAZ ÖZTÜRK, İ̇lknur DAĞ

Konular:Tıp

Anahtar Kelimeler:Metformin,Lver,TEM

Özet: Metformin is the most commonly prescribed oral anti-diabetic agent for type II diabetes. It is generally considered as a safe drug but it is reported that it may also have a hepatotoxic effect. Studies in the literature evaluating the morphological and ultrastructural effects of metformin on liver tissue are more likely include whether it reduces an experimentally created liver injury. However, this drug can also be used in patients without any liver disease, such as polycystic ovarian syndrome. Our aim in this study is to evaluate whether metformin use causes any change in the liver with the help of light and permeable electron microscopy (TEM) while there is no liver disease. For this purpose, Wistar Albino male rats were divided into two groups as control and metformin groups. Metformin (100mg/kg) was applied to the metformin group for three weeks and an equal volume of saline was given to the control group. Liver samples were taken from rats under ketamine/xylazine anesthesia after one week of the drug-free period and euthanasia was performed. After biopsy, liver samples were taken for routine TEM procedure for light and electron microscopic examinations. Semithin (700 nm) and ultrathin (60 nm) sections from samples were evaluated by firstly light microscope and then Hitachi HT 7800 TEM. While healthy findings including regular hepatocyte, nucleus and nucleolus were observed in the control group samples, abundant vacuol formation, nuclear irregularity and shrinkage, abundant blood vessel and hepatocyte separation were observed in metformin-treated samples. The findings of our study show that metformin may have adverse effects on hepatocytes in long-term use.

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