Eurasian Journal of Biological and Chemical Sciences

Yazarlar: Aykut TOPDEMİR, Nazmi GÜR, Zümre DEMİR

Konular:Fen

Anahtar Kelimeler:Flavanoid,Melissa officinalis L.,Phenolic compound,Antioxidant,Callus

Özet: Lemon grass (Melissa officinalis L.) is a species used as a medicinal and aromatic plant, marketed on the domestic market and also exported. Lemon grass has many uses in the pharmaceutical, perfumery, cosmetics and food industries. The prominence of M. officinalis is due to the essential oils found in citronelal and citral. In addition to these two components, compounds such as linalool, geraniol, ά-pinene and terpinene are contained at a lower rate. Callus cultures and cell suspension cultures are in vitro propagation pathways for medical and aromatic plants. It is possible to obtain metabolites of plant secondary in the same quality and in high quantity. In this study, total phenolic substances and flavanoid amounts of calli produced in vitro were determined. M. officinalis nodes were used as explant source. Nodules were promoted with different plant growth regulator combinations for callus formation in Murashige Skoog medium. The highest phenolic substance content was determined from 1523 mg / g to 1.5 mg / L 2,4-D + 0.5 mg / L BAP-induced callus. 1.356 mg / g and the lowest phenolic substance was found in cultured calli containing 2 mg / L 2,4-D + 0.5 mg / L BAP. In the culture medium with the highest amount of flavanoid (4.392 mg / g), there is a plant growth regulator of 2 mg / L 2,4-D + 1 mg / L PIC + 0.5 mg / L KIN..

---