Eurasian Journal of Biological and Chemical Sciences

Yazarlar: Muhammet DOGAN

Konular:Biyoloji

Anahtar Kelimeler:Tissue culture,Light effect,LED,Shoot regeneration

Özet: Since light emitting diodes (LEDs) have important advantages for plant growth and development, they have been used in tissue culture studies. The light colours of LEDs show different effects on plants. In this study, single and combined effects of white (W), red (R), blue (B) LED lights on multiple shoot regeneration of Lysimachia nummularia L. were investigated. Nodal explants were cultured for eight weeks in a Murashige and Skoog (MS) nutrient medium supplemented with 0.10 mg/L indole butyric acid (IBA) and 0.20-0.80 mg/L Thidiazuron (TDZ). 100% shoot regeneration frequencies in all three hormone applications were recorded under 2W:1R: 1B and 1W: 1R: 1B LED lights. The maximum number of shoots was determined under 1W:2R:1B LED lights as 17.47, 16.16 and 13.46 shoots/explant in the MS nutrient medium added 0.40 mg/L TDZ + 0.10 mg/L IBA, 0.20 mg/L TDZ + 0.10 mg/L and 0.80 mg/L TDZ + 0.10 mg/L IBA, respectively. The minimum number of shoots was recorded under blue LED. The longest shoot was obtained from explants under 1W:1R:2B LED light in all environments. Excessive use of TDZ in the culture medium adversely affected shoot length. Since there was a dense root in replication experiments, no rooting was performed. Plants produced in the culture environment have been successfully adapted to external conditions. As a result, the study showed the effectiveness of the LED lights on the production of L. nummularia and their superiority over the fluorescent lights.

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