Sağlık Bilimlerinde İleri Araştırmalar Dergisi

Yazarlar: Çiğdem KEKİK ÇINAR, Gonca KARAHAN, Sonay TEMURHAN, Sevgi KALAYOĞLU BEŞIŞIK, Fatma Savran OĞUZ OĞUZ, Filiz AYDIN

Konular:Tıp

Anahtar Kelimeler:Cytokine,Polymorphism,Multiple Myeloma,Chromosome 13,Deletion

Özet: Objective: Multiple myeloma (MM) is a clonal enlargement of plasma cells. The most common cytogenetic anomaly in MM is monosomy 13 and appears approximately 40%–50% in multiple myeloma. Studies have shown that in the majority of patients, 80%–90% of chromosomal anomalies are monosomy 13, and 10%–20% are regional deletions. Monosomy 13 is the most powerful predictor of survival in MM. Cytokines are proteins that regulate many functions of these cells and are secreted by cells of the immune system. Plasma cell stimulates angiogenesis by increasing the release of cytokines such as IL-1β, IL-6, and IL-10. Single nucleotide polymorphisms (SNP) of cytokine genes can affect their secretion rate or biological activity. Two of the important factors for MM disease are cytokines and chromosome 13 deletion (del13). We investigate chromosome 13 deletion and cytokine gene polymorphisms (IFN-γ, TGF-β, TNF-α, IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, IL-12, IL-1R, IL-1Rα, IL-4Rα) in patients with MM. Materials and Methods: EDTA blood was collected from 38 patients with MM included in the study, and DNA was isolated. PCR-SSP method was used for cytokine gene polymorphism typing (Protrans). FISH method was applied using LSI D13S25 SO DNA probe for deletion 13. Results: 13q deletion was detected in 15 of the 38 of the patients. Statistical analysis of 13q deletion and cytokine gene polymorphisms was not statistically significant (p>0.05) by patient age, sex, disease stage, and β2-microglobulin levels. There is a statistical significance between level of LDH and TGF-β TG/TG haplotype (p=0.002). No significant results were obtained in the relationship between cytokine polymorphisms and del13 (p>0.05). Conclusion: Cytokines, which are associated with the function and viability of plasma cells, have a major role in the etiology and prognosis of the disease. Moreover, similar effects were observed with the 13q deletion. In our study, we did not find a statistical significance between cytokine gene polymorphisms and 13q deletion. Although both parameters independently have great importance on the effects of the disease, combining them together does not yield the same effect.

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