Sağlık Bilimlerinde İleri Araştırmalar Dergisi
Yazarlar: Selin COŞKUN, Şükriye YILMAZ, Ayşe ÇIRAKOĞLU, R. Dilhan KURU, Yelda TARKAN ARGÜDEN ARGÜDEN, Şeniz ÖNGÖREN, Seniha HACIHANEFİOĞLU
Konular:Tıp
Anahtar Kelimeler:Myelodysplastic syndrome,Cytogenetics,Interphase FISH
Özet: Objective: Chromosome abnormalities are observed in 30-50% of myelodysplastic syndrome (MDS) cases. The most common anomalies are trisomy 8, monosomy 7 / 7q-, monosomy 5 / 5q- and 20q-. Conventional cytogenetic and interphase FISH methods are used to detect these anomalies. Although bone marrow is the preferred material for both methods, the suitability of using peripheral blood is also being investigated. The aim of this study is to contribute to the data pool in this area by presenting comparative cytogenetic and interphase FISH examination results in peripheral blood and bone marrow samples of MDS patients who applied to our laboratory. Materials and Methods: Peripheral blood and bone marrow samples of 19 patients with MDS were examined with conventional cytogenetic and iFISH methods using deletion probes specific to the 5q31, 7q22 and 7q31 regions. The data obtained were used to compare the materials and techniques. Results: Conventional cytogenetics: In this technique, the results of peripheral blood and bone marrow samples were concordant in 5 cases (normal karyotypes in 2, and abnormal in 3 cases), and discordant in 12 cases, and in two cases, there was no metaphase in bone marrow samples, while abnormal karyotypes were observed in blood samples. iFISH: Between the results of peripheral blood and bone marrow; for -5/del(5q) there was concordance in 13 cases (positive in two, and negative in three cases), and for -7/del(7q), 10 cases were concordant (positive in one, and negative in 9 cases). The one case that del(5q) and del(7q) were observed together in conventional cytogenetic examination, was positive in iFISH analysis for both anomalies, too. Conclusion: When we evaluate our results together with previous reports, it seems that both methods and both materials have their own advantages and disadvantages. Therefore, we suggest that it would be beneficial to use these two methods and materials in parallel instead of replacing each other.