Sağlık Bilimlerinde İleri Araştırmalar Dergisi

Yazarlar: Ayşe Berna YÜZBAŞIOĞULLARI, Bilge ÖZSAİT SELÇUK, Evrim KÖMÜRCÜ BAYRAK, Nihal ERGİNEL ÜNALTUNA

Konular:Temel Bilimler

DOI:10.26650/JARHS2019-651072

Anahtar Kelimeler:SiRNA,H9c2,Tbn,Pparg,Taf10

Özet: Differential gene expression is important in the regulation and maintenance of developmental stages and tissue specific physiological processes. The subtractive hybridization method is an efficient method for the detection of genes that have tissue specific expressions. In our previous work, our team had constructed a BALB/c mice heart tissue specific subtractive hybridization cDNA library. Taube Nuss (Tbn), which is a crucial transcription factor, is one of the genes that were isolated from this library. In this study, our aim was to analyze the expression of possible Tbn related genes and identify the Tbn pathway. In the scope of this study, the Tbn gene expression in BALB/c mice heart and skeletal tissue was analyzed with the Northern Blot technique. Then, the Tbn gene was silenced in H9c2 rat cardiomyoblast cell line using siRNA specific to the TBN gene. The gene expression levels of Pparg, Cebpa, Taf10 and Myocd genes, which were possibly related to the Tbn pathway, were analyzed in silenced cells. We observed that the expression of the Myocd gene was decreased, whereas the expression of the Pparg gene was increased in these cells. These results suggest that the Tbn gene plays a regulatory role in cardiac tissue. 

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