Ziraat Mühendisliği

Yazarlar: Hüseyin UYSAL, Tuğba TOPBAŞ

Konular:Ziraat, Bitki Bilimleri, Ziraat, Ortak Disiplinler

DOI:10.33724/zm.888511

Anahtar Kelimeler:Sainfoin,Onobrychis viciifolia,In vitro,Auxin,Cytokinin

Özet: This study was carried out to investigate the development of hypocotyl explants of the sainfoin (Onobrychis viciifolia Scop.) Özerbey-03 variety in nutrient media containing BAP (6-benzyl amino purine-cytokinin hormone) and IAA (3-indole acetic acid-auxin hormone). In the study, MS (Murashige and Skkog) medium was used as the main medium and 0.5 mg.l-1 BAP and IAA were added separately, and the cytokinin and the auxin hormones were added together in the ratio of 0.5 mg.l-1 BAP + 0.5 mg.l-1 IAA, 0.5 mg.l-1 BAP + 0.1 mg.l-1 IAA and 0.1 mg.l-1 BAP + 0.5 mg.l-1 IAA to this medium. As a result of the study, it was determined that the best result (84%) was gained in terms of direct regeneration from the medium without using any hormones, and the best result (84%) was gained in terms of callus formation from the medium containing 0.5 mg.l-1 BAP + 0.1 mg.l-1 IAA, when the mediums are evaluated in terms of the total regeneration, the best result (100%) was given by the medium containing 0.5 mg.l-1 BAP + 0.1 mg.l-1 IAA and 0.1 mg.l-1 BAP + 0.5 mg.l-1 IAA. In addition, the best result (0.83 pcs / callus) in terms of shoot regeneration from calluses was obtained from the medium containing 0.5 mg.l-1 BAP. As a general result of this research, it can be said that the preference of hormone-free medium for direct regeneration and the medium containing high concentrations of BAP and BAP + IAA in terms of callus formation will increase the success rate in sainfoin plants.

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