Journal of Apitherapy and Nature

Yazarlar: Wallance M. PAZIN, Vita SOLOVYEVA, Tibebe LEMMA, Bjarke JØRGENSEN, Carlos José L. CONSTANTINO, Jonathan BREWER

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Anahtar Kelimeler:Fibroblast Cells,CARS Microscopy,Brazilian grepropolis

Özet: Artepillin C, the major component of the Brazilian green propolis, has become the subject of great biological interest among all the secondary metabolites found in that propolis, especially due to its anticancer property. Biophysical studies have shown that the presence of two prenylated groups in the molecular structure of Artepillin C enhances its affinity for lipophilic environment 1 , which is in agreement with biological assays reported in the literature, suggesting that Artepillin C is absorbed through cell membranes 2 . The purpose of this study was to obtain for the first time a direct visualization of Artepillin C internalization into fibroblast cells by means of Coherence Anti-Stokes Raman Scattering (CARS), a powerful microscopy technique with a combination of two pulsed laser beams which induces a vibrational motion of a specific compound, generating an intrinsic image 3 . A detailed vibrational characterization of Artepillin C has been carried out using Raman microspectroscopy and surface-enhanced Raman scattering (SERS) in combination with quantum chemical calculation based on LANL2DZ/B3LYP level of theory.  The success use of CARS microscopy was confirmed by collecting Artepillin C images from dry and hydrated films of the compound, while the intrinsic images of Artepillin C internalized into the cells was compared to confocal fluorescence microscopy images by using a cell-membrane staining compound. With effect, this study shows for the first time a direct visualization of the major compound of green propolis into the cells by means of CARS microscopy.   Acknowledgements : FAPESP (2017/23426-4) and DaMBIC (University of Southern Denmark).

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