Asian Pacific Journal of Health Sciences

Yazarlar: Ahmed Tabbabi, Ali Lamari, Ben Haj Ayed Ahlem, Raja Ben Cheikh, Hassen Ben Cheikh, Jaber Daaboub

Konular:-

DOI:10.21276/apjhs.2017.4.2.26

Anahtar Kelimeler:Culex pipiens,Temephos resistance,Detoxification mechanisms,Target site alteration,Northern Tunisia

Özet: In order to understand the resistance of Culex pipiens to temephos and provide parameters for management programs, we evaluated the susceptibility levels to temephos of individuals collected in five localities of Northern Tunisia. Our results showed that sample # 2 was susceptible. The resistant samples displayed RR50 ranged from 1.3 in sample # 5 to 440 in sample # 4. Mortality caused by propoxur ranged from 0% in sample # 4, which showed the highest resistance levels to studied temephos insecticide and indicated an important contribution of AChE 1, to 68% in sample # 5. Starch gel electrophoresis identified many esterases in studied samples with an important frequency (85%) in the sample # 4. This sample showed the highest resistance to temephos with a major contribution of CYP450, esterases, and AChE 1. Both detoxification mechanisms and target site alteration were involved in the resistance to temephos as reported in our study. This is not a new phenomenon in mosquitoes, in which multiple insecticide resistance mechanisms has been reported worldwide.

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