Natural Products and Biotechnology

Yazarlar: Mücahit Seçme, Yavuz Dodurga

Konular:-

Anahtar Kelimeler:Usnic acid,Long,On coding RNA,UCA1,Ishikawa cells,Endometrial cancer

Özet: Endometrial cancer is the most common gynecological cancer in developed countries. Gene expressions in cancer cells are known to be controlled by a wide array of regulatory molecules including non-coding RNAs. Noncoding RNAs contribute to many cellular events in the cancer mechanisms including proliferation, apoptosis, angiogenesis, invasion, migration and metastasis. UCA1 has been defined as an oncogene due to its effect on cellular mechanisms such as cancer cell invasion, migration, proliferation and metastasis in different types of cancers including colon, lung, breast, bladder, endometrial gastric and pancreatic cancer. The effects of usnic acid on cell proliferation in Ishikawa endometrial cancer cells were detected by XTT assay. Total RNA was isolated by Trizol-reagent and subsequently total transcriptome cDNA was synthesized. LncRNA UCA1 expression change between control and usnic acid treated groups was determined by the Real-Time PCR method. Usnic acid decreases cell proliferation and its IC50 value in Ishikawa cells was detected as 51,76 µM at 48th hour. UCA1 expression was downregulated in usnic acid treated Ishikawa cells in vitro. According to results, usnic acid inhibits the proliferation of Ishikawa cells and its effect mechanisms may be associated by reducing the expression of UCA1 oncogenic characteristic long noncoding RNA that have a role in different cellular mechanisms in cancer. In conclusion, this study may contribute further and detailed studies about usnic acid and its therapeutic potential for endometrial cancer treatment.

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