Natural Products and Biotechnology

Yazarlar: Canan Eroğlu Güneş, Fatma Seçer Çelik, Mücahit Seçme, Ercan Kurar

Konular:-

Anahtar Kelimeler:Apoptosis,Invasion,Melanoma cells,Metastasis,Oleandrin

Özet: Skin cancer has an increasing incidence worldwide. Non-melanoma skin cancers and malignant melanomas are the most common skin malignancy. Nerium oleander L., which is a member Apocynaceae family, has historically been used in the treatment of hemorrhoids, leprosy and snake bites. Oleandrin is one of the cardiac glycosides obtained from N. oleander. The study aimed to evaluate the anticancer effects of oleandrin in A375 human melanoma cells via apoptosis, metastasis and invasion pathways. The effect of oleandrin on cell viability was evaluated using an XTT cell viability assay. Expressions of 8 genes in apoptosis and 10 genes in metastasis and invasion were determined by RT-qPCR. The IC50 dose of oleandrin was found to be 47 nM for 48 h in A375 melanoma cells using CompuSyn version 1.0 software. Oleandrin treatment significantly increased the expression of CASP9, FAS, CYCS, CDH1 and TIMP3; however, significantly decreased the expression of BCL2, P53, CDH2 and TGFB3 in A375 cells. In conclusion, changes in expression levels of apoptosis, metastasis and invasion genes indicated that oleandrin has an apoptotic and anti-metastatic effect in A375 cells.

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