A Journal of Analytical Chemistry and Chemical Analysis

Yazarlar: Toshiki Horikoshi, Chihiro Kitaoka, Yosuke Fujii, Takashi Asano, Jiawei Xu, Tatsuya Fujino

Konular:-

DOI:10.3390/analytica2030008

Anahtar Kelimeler:Matrix-assisted laser desorption ionization (MALDI),Surface-assisted laser desorption ionization (SALDI),Fingerprint,Acetaminophen,Zeolite

Özet: The ingredients of an antipyretic (acetaminophen, AAP) and their metabolites excreted into fingerprint were detected by surface-assisted laser desorption ionization (SALDI) mass spectrometry using zeolite. In the fingerprint taken 4 h after AAP ingestion, not only AAP but also the glucuronic acid conjugate of AAP (GAAP), caffeine (Caf), ethenzamide (Eth), salicylamide (Sala; a metabolite of Eth), and urea were detected. Fingerprints were collected over time to determine how the amounts of AAP and its metabolite changed with time, and the time dependence of the peak intensities of protonated AAP and GAAP was measured. It was found that the increase of [GAAP+H] + peak started later than that of [AAP+H] + peak, reflecting the metabolism of AAP. Both AAP and GAAP reached maximum concentrations approximately 3 h after ingestion, and were excreted from the body with a half-life of approximately 3.3 h. In addition, fingerprint preservation was confirmed by optical microscopy, and fingerprint shape was retained even after laser irradiation of the fingerprint. Our method may be used in fingerprint analysis.

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