A Journal of Analytical Chemistry and Chemical Analysis

Yazarlar: Eleni Doulou, Marina Kalomiraki, Anthi Parla, Kyriaki Thermos, Nikos A. Chaniotakis, Irene Panderi

Konular:-

DOI:10.3390/analytica2040012

Anahtar Kelimeler:Hydrophilic interaction liquid chromatography,Fluorescence detection,Octreotide,Octreotide acetate,SMS 201-995,Somatostatin

Özet: Octreotide is a synthetic cyclic octapeptide analogue of somatostatin-14. It is mainly administered for the treatment of acromegaly, severe diarrhea, and neuroendocrine neoplasias. In this work, a hydrophilic interaction liquid chromatography (HILIC) method with fluorescence (FL) detection was developed and validated for the quantitation of octreotide in solutions for injection. Chromatographic separation was performed on an XBridge®-HILIC analytical column under isocratic elution with a short chromatographic run time of less than 10 min. The mobile phase consisted of ammonium bicarbonate 8.6 mM (pH 8.1)/acetonitrile 35/65 ( v/v). The high sensitivity and selectivity of the fluorescence detection, with the excitation wavelength (λ excitation) set at 280 nm and the emission wavelength set at (λ emission) 330 nm, enabled a simple sample preparation procedure that included only dilution steps. The calibration curve showed good linearity with a correlation coefficient greater than 0.998. The method was successfully applied to the analysis of commercially available octreotide injection forms.

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