A Journal of Analytical Chemistry and Chemical Analysis

Yazarlar: Antonio Marín-Romero, Mavys Tabraue-Chávez, Bárbara López-Longarela, Mario A. Fara, Rosario M. Sánchez-Martín, James W. Dear, Hugh Ilyine, Juan J. Díaz-Mochón, Salvatore Pernagallo

Konular:-

DOI:10.3390/analytica2040013

Anahtar Kelimeler:Dynamic chemical labelling (DCL),Drug-induced liver injury (DILI),MiRNA-122,Luminex MAGPIX,Liquid biopsy,Antibody-dependant method

Özet: Drug-induced liver injury (DILI) is a potentially fatal adverse event and a leading cause for pre- and post-marketing drug withdrawal. Several multinational DILI initiatives have now recommended a panel of protein and microRNA (miRNA) biomarkers that can detect early liver injury and inform about mechanistic basis. This manuscript describes the development of seqCOMBO, a unique combo-multiplexed assay which combines the dynamic chemical labelling approach and an antibody-dependant method on the Luminex MAGPIX system. SeqCOMBO enables a versatile multiplexing platform to perform qualitative and quantitative analysis of proteins and miRNAs in patient serum samples simultaneously. To the best of our knowledge, this is the first method to profile protein and miRNA biomarkers to diagnose DILI in a single-step assay.

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