A Journal of Analytical Chemistry and Chemical Analysis

Yazarlar: Petra Valigurová, Lenka Jarešová, Jana Váňová and Petr Česla

Konular:-

DOI:10.3390/analytica1010004

Anahtar Kelimeler:Amino acid surfactants,2,′-dibromoacetophenone,Derivatization,Reversed phase liquid chromatography,Gradient elution

Özet: A method for the analysis of weak anionic surfactants based on N-acyl amino acids was developed. The surfactants were derivatized using 2,4′-dibromoacetophenone yielding 4′-bromophenacyl esters suitable for spectrophotometric detection. Surfactants containing glycine, threonine and glutamic acid were analyzed after derivatization using reversed-phase liquid chromatography with UV/Vis and MS detection. The gradient profile was optimized using isocratic retention data of N-acyl-linked fatty acid homologues. The relative content of the homologues of N-acyl-linked fatty acids was expressed using the determined method. The intraday repeatability and stability of the prepared derivatives was tested. The relative content of fatty acids in the surfactants was correlated with the most common sources of fatty acids, showing high Pearson’s correlation coefficients with the typical fatty acids profile of a coconut oil.

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