Uluslararası Fen Araştırmalarında Yenilikçi Yaklaşımlar Dergisi

Yazarlar: Fatih Sezer

Konular:-

DOI:10.29329/ijiasr.2019.187.1

Anahtar Kelimeler:SCARECROW,SCR,Olive,GRAS,Transcription factor

Özet: The Olive (Olea europaea L.) is a Mediterranean crop tree with great economic importance and understanding molecular mechanisms that control developmental processes like adventitious rooting will benefit various aspects of olive tree development research. SCARECROW (SCR) genes are reported to be essential for asymmetric division of the cortex/endodermis progenitor cell in the root. They also have diverse roles through plant development. Thus, this study aimed to identify SCR genes in Olive. With this study we investigated all GRAS family proteins in Olive and further extracted SCR proteins in Olive genome. With this study, we report SCR genes in olive for the first time and provide analysis of their structure with bioinformatic tools. Phylogenetic tree revealed that all 8 major sub-families of GRAS were present in olive genome and some other sub-families like SCL18, SCL3 and SCL28 were also present. A. thaliana SCR (AT3G54220) was grouped with 2 unique olive sequences (XP_022893717.1, XP_022881217.1). Both sequences showed a 2 exon, 1 intron structure and contained the characteristic GRAS domain. Promoter regions contained several light and low temperature motifs. Auxin, gibberellin and abscisic acid response elements were present within these regions. Promoter regions of all olive SCR also contained MYB transcription factor recognition and binding sites.

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