Current Proceedings on Technology

Yazarlar: Robab Davar, Reza Darvishzadeh, Sodabeh Jahanbakhsh Godehkahriz

Konular:-

Anahtar Kelimeler:Basal stem rot,Helianthus annuus L.,Interaction effect slicing,SAS software,Sclerotinia sclerotiorum.

Özet: Sunflower, Helianthus annuus L. is a major oilseed crop widely cultivated throughout the globe. White mold, caused by Sclerotinia sclerotiorum, is a common and widespread pathogen of sunflower. Thirty five sunflower genotypes were selected on the basis of their agricultural characteristics. Genotypes, including recombinant inbred lines and their parents, M7 mutant lines developed by gamma irradiation, and some genotypes from different geographical origins, were inoculated with eight different S. sclerotiorum isolates in controlled conditions. A factorial experiment was arranged in completely randomized design with six replications. The percentage of the subsequent basal stem lesions was measured 3 days after inoculation. The normality of disease severity data  were  assessed  with  the Shapiro-Wilks  test  (Proc  Univariate;  SAS  Institute  Inc., Cary, NC, USA). The main effect of genotypes and isolates as well as their interactions were determined. Line × isolate interaction effect was sliced by isolate in the SAS software in order to identify the specificity of sunflower lines to particular isolate. S. sclerotiorum isolates interacted differentially with sunflower genotypes. For example, Mutant line M7-575-1 presented high partial resistance to all S. sclerotiorum isolates, whereas SDB3 lines were susceptible to all isolates. The least and most aggressive isolates were SSU35 and SSKH26, respectively. Some lines had specific interactions with S. sclerotiorum isolates, such as RHA265 which had specific resistance to SSU73 isolate but was more susceptible to most other isolates. The information presented here could assist sunflower breeders to choose parents of crosses for breeding of durable resistance to S. sclerotiorum basal stem disease. 

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