Current Proceedings on Technology

Yazarlar: Robab Davar, Reza Darvishzadeh, Sodabeh Jahanbakhsh Godehkahriz

Konular:-

Anahtar Kelimeler:Aggressiveness,ANOVA,Helianthus annuus L.,SAS software,Sclerotinia sclerotiorum.

Özet: Basal stem rot, caused by Sclerotinia sclerotiorum (Lib.) de Bary, is an important cause of yield loss in sunflower (Helianthus annuus L.). The main aim of the present study was to determine the aggressiveness of isolates of S. sclerotiorum on a single cultivar of sunflower using statistical methods. The aggressiveness of 108 isolates was determined on the sunflower hybrid cv. Iroflor. Plants were grown in a controlled condition. The experiment was arranged in a completely randomized design with three replications per isolate. The normality of the disease severity data was assessed in SAS (PROC UNIVARIATE; SAS Institute Inc., Cary, NC, USA). Analysis of variance (ANOVA) of the disease severity data was performed using the general linear model (GLM) procedure in the SAS software. The inoculation of sunflower stems with mycelial plugs and the measurement of lesion development were used to compare aggressiveness between isolates. Isolates of S. sclerotiorum differ significantly in aggressiveness on sunflower. Rank ordering of isolates indicated differences and lesion percentage ranged from 8 to 100 % depending to isolates. Screening of the aggressiveness of S. sclerotiorum isolates would be best conducted before their use in resistance screening to ensure that hypovirulent isolates are not used in resistance screening. 

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