Türk Bilimsel Derlemeler Dergisi

Yazarlar: Serdar YÜKSEL, Özgür EROĞLU

Konular:-

Anahtar Kelimeler:Clinical cytogenetics,Karyotype,Cell culture,Chromosome staining

Özet: Clinical cytogenetics, production of human bone marrow cells, fibroblast cells and lymphocyte cells in vitro, preparation of microscopepreparations and stopping in the metaphase stage this cells, staining of these preparations, obtaining of chromosomal bands and quantitative andstructural evaluation of human chromosomes can be summarized. Clinical cytogenetic studies involve processes that are sensitive, sometimeseven influenced by climate and regional conditions, which are simply summarized by a simple cell culture study following a simple cell culturestudy, but in fact require laborious, patient, expert and experienced laboratory personnel. Therefore, it is difficult to obtain sufficient and highquality metaphase area in cytogenetics. For this reason, we mentioned in this study what to do in order to obtain metaphase field in cytogeneticsin sufficient number, quality and high band resolution. The most effective methods and the points to be noted in these steps were determinedby comparing the staining procedures of cord blood, peripheral blood cell culture with cell culture stages. If the sample is studied in clinicalcytogenetics, the most critical stage of the study is the cell culture stage. Addition of methanol-acetic acid is particularly important for thesampling of the sample, the state of the sample, the stage of sowing, the growth and infection in the post-sowing incubator, the prevention ofprecipitation in the incubator in the blood culture.

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