Journal of Medicine and Palliative Care

Yazarlar: Duygu KIRKIK, Sevgi KALKANLI TAŞ, Eylem ÇAĞILTAY, Nevin KALKANLİ

Konular:Sağlık Bilimleri ve Hizmetleri

DOI:10.47582/jompac.739257

Anahtar Kelimeler:CTLA-4,In silico,MiRNA,SNP,Biyoinformatik

Özet: Introduction: CTLA-4 (cytotoxic T-lymphocyte associated antigen-4) gene is a member of the immunoglobulin gene superfamily, encoding the protein that transmits an inhibiting signal to T cells. The CTLA-4 gene is an immune checkpoint  among the genes being investigated  nowadays. The aim of this study was to find  various polymorphisms in the CTLA-4 gene may contribute to the pathogenesis of autoimmune thyroid disease (AITD) by investigating the genes that are similar to the CTLA-4 gene. Material and Method: In our study, we used GeneMania and STRING databases to find similar genes with CTLA-4 gene, then we used Polyphen2, Exome Variant Server and SIFT databases to find single nucleotide polymorphisms (SNPs), miRDB and miRWalk databases were used to detect micro RNAs in the CTLA-4 gene. According to our results, we found that the FYN gene may contribute to the pathogenesis of AITD.  Results: We  discovered SNPs (rs201778935, rs138279736, rs369567630 and rs376038796) that are associated with the CTLA-4 gene. In our study, we have thought that 31 miRNAs are therapeutically important, they play a regulatory role in the production of proteins by messenger RNAs. Conclusion: We believe that this study, which we evaluated with bioinformatics methods, can contribute to  the explanation of  the pathogenesis of many disease by performing lab based experiments in the future.

---