The Journal of Neurobehavioral Sciences

Yazarlar: Tela dris Abdu, Musa Sunday Abraham, Iliya Ibrahim Abdullahi, Nzalak James Oliver

Konular:-

Anahtar Kelimeler:-

Özet: The study was designed to determine whether lactational exposure to hyoscyamine fraction of Datura stramonium L. (D. stramonium) seeds affect the cognitive, spatial learning and memory functions of the hippocampus in Wistar rats at adulthood. Fresh seeds of D. stramonium L. were procured, macerated and fractionated using high-performance liquid chromatography (HPLC). Eight (8) Wistar rats weighed 150-250 grams of equal gender were used for the study. The rats were mated and divided into control and treatment groups. Equivalent body weight of normal saline and 400 mg/kgbwt of hyoscyamine fraction were orally administered to the breastfeeding rats respectively on lactational days (LD) 1-21. At adulthood, the rats were subjected to neurobehavioural tests using novel objects recognition (NORT) and Morris water maze (MWM) respectively. The data obtained were expressed as mean ± SEM, independent two samples t-test and General Linear Model (GLM) repeatedmeasures ANOVA with Fisher’s multiple comparisons post-hoc tests were used to show the mean differences using Minitab 17 (LLC., U.K.) statistical package software. P < 0.05 was considered statistically significant. A significant increase in the meantime of exploration between the groups (p = 0.049) was observed during the NORT. No statistically significant increase (p = 0.626) in the meantime to locate the escape platform between the groups during the MWM test. The CA1 region of the treated group showed mild nuclear hyperchromasia, cytoplasmic vacuolations and pyknosis. In conclusion, exposure to hyoscyamine fraction of D. stramonium L. seeds at lactation caused histologic changes in the CA1 region, loss in short-term memory but not spatial learning and memory functions of the hippocampus of Wistar rats at adulthood.

---