Journal of Applied Biological Sciences

Yazarlar: Aynur Aybey, Elif Demirkan, İsmail Alper Susurluk

Konular:-

Anahtar Kelimeler:Bacillus thuringiensis,Antibacterial substance,Crystal,Nematicidal activity,Meloidogyne incognita

Özet: This work represents to investigate the capacity to synthesize antibacterial substances and nematicidal effect from Bacillus thuringiensis (Bt) strains isolated from agricultural soil samples of Turkey. Isolated Bacillus strains were identified based on 16S rRNA sequence analysis. The four Bt isolates showed 100 % sequence identity with Bt. These strains were named as Bt A1, A14, A15 and A17. The Bt strains were tested for resistance to ampicillin, streptomycin, bacitracin, penicillin, tetracycline. While all Bt strains were sensitive to tetracycline and streptomycin, they showed resistance to ampicillin, penicillin and bacitracin. These strains were also analyzed using the agar diffusion method in terms of their antibacterial activities to some pathogen bacteria. It has been found that Bt A14 isolate was effective against both of some gram negative and positive bacteria. Especially, it was observed to be the effective against K. pneumoniae and Y. enterocolitica as gram positive bacteria. In addition, supernatants and pellets of Bt strains were investigated for the root-knot nematode Meloidogyne incognita. Both of them were found as a good nematicidal agents

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