Journal of Applied Biological Sciences

Yazarlar: Nevra Pelin CESUR, Volkan YALMAN, Melisa TÜRKOĞLU LAÇİN

Konular:-

Anahtar Kelimeler:Decellularization,Supercritical carbon dioxide (scCO2),Cow aorta tissue

Özet: Decellularized tissues have been extensively studied in recent years thanks to functioning as potential tissue scaffolds consisting of natural extracellular matrix components (ECM). The most critical issue to obtain a decellularized tissue is the removal of cells on the ECM without destroying the ECM components. Besides, while decellularizing a  tissue, keeping the ultra-structure of the 3D composition of native ECM and thus, saving mechanical behavior is essential. In this study, the decellularization of cow aorta with SFE and with a hybrid method combining TritonX-100 / SFE was compared. The aorta was treated by SFE for 1h, 4h, 6h, and a combined method (SFE/Triton X-100) was performed as 2 hours Triton-X-100/1-hour SFE treatment and, overnight Triton-X-100 /1h SFE treatment. The complex structure of the ECM after decellularization via SFE was obtained fairly similar to native ECM compared to TritonX-100 treated cow aortic tissue samples according to the SEM images and H&E staining results. At the same time, tissue samples treated with SFE demonstrated an equal decellularization yield in a shorter time than those decellularized with chemical methods. Besides, mechanical strength and protein integrity of decellularized ECM by SFE was determined similar to the native tissue

---