Journal of Applied Biological Sciences

Journal of Applied Biological Sciences

Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp.

Yazarlar: Ebrahim VALIPOUR, Yasemin CAF, Burhan ARIKAN

Cilt 8 , Sayı 3 , 2014 , Sayfalar 01-05

Konular:-

Anahtar Kelimeler:Bacillus sp,Cold–adaptive cellulase,Detergent,Energy saving

Özet: A cold-adaptive acidic cellulase enzyme was produced by native Bacillus sp. TG-1 from Adana-TURKEY and the cellulase producing bacteria and the enzyme was characterized. In SDS-PAGE analysis the enzyme showed single bond of 52.4 kDa. The cellulase TG-1 had a pH optimum of 4.6 and displayed maximum activity at 45ºC. The enzyme was stable at pH ranging from 4.0 to 9.0 with more than 85% of original activity for 24 h. Also, the enzyme showed 100% stability at 20-40ºC for 60 min and exhibited more than 63% of maximum activity in different NaCl concentrations. The enzyme showed up to 70% stability against detergents and chelators, while it was strongly inhibited by Urea, H2O2, PMSF and 1,10-Phenanthroline and induced by β-mercaptoethanol and MnCl2. TLC analyses of the hydrolysis of Carboxymethyl cellulose (CMC) by the enzyme, revealed the presence of maltose and etc.

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@article{2014, title={Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp.}, volume={8}, number={01–05}, publisher={Journal of Applied Biological Sciences}, author={Ebrahim VALIPOUR, Yasemin CAF, Burhan ARIKAN}, year={2014} }
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Ebrahim VALIPOUR, Yasemin CAF, Burhan ARIKAN. (2014). Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-adaptive Cellulase (CMCase) from a Native Isolate Bacillus sp. (Vol. 8). Vol. 8. Journal of Applied Biological Sciences.
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Ebrahim VALIPOUR, Yasemin CAF, Burhan ARIKAN. Isolation and Characterization of a Novel Acidophilic, Halotolerant and Cold-Adaptive Cellulase (CMCase) from a Native Isolate Bacillus Sp. no. 01–05, Journal of Applied Biological Sciences, 2014.
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