Journal of Applied Biological Sciences

Journal of Applied Biological Sciences

Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli

Yazarlar: Lawrance ANBU RAJAN, Yeragudipati RAJALAKSHMI, Vetrivel UMASHANKAR

Cilt 3 , Sayı 3 , 2009 , Sayfalar 55-59

Konular:-

Anahtar Kelimeler:Trehalose-6-phosphate synthase,Trehalose-6-phosphate phosphatase,Compatible solutes,Salt stress

Özet: Trehalose, a non-reducing disaccharide is a major compatible solute, maintains fluidity of membranes and protects the biological structure of the organisms under stress. In this study, trehalose-6-phosphate synthase (TPS) and trehalose-6-phosphate phosphatase (TPP) genes encoding for trehalose biosynthesis were PCR amplified from genomic DNA of Escherichia coli isolated from Rohu (Labeo rohita) collected from local fish market of Cochin, Kerala, India. The amplified genes were cloned and nucleotide sequences were determined. The sequencing results showed that (TPS) and (TPP) genes contain1,424 bp and 801 bp long ORF encoding 474 and 266 amino acids respectively. In silico sequence analysis revealed that the (TPS) and (TPP) sequences of E. coli were conserved in many eubacteria.

ATIFLAR
Atıf Yapan Eserler
Henüz Atıf Yapılmamıştır
KAYNAK GÖSTER
BibTex
KOPYALA
@article{2009, title={Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli}, volume={3}, number={55–59}, publisher={Journal of Applied Biological Sciences}, author={Lawrance ANBU RAJAN, Yeragudipati RAJALAKSHMI, Vetrivel UMASHANKAR}, year={2009} }
APA
KOPYALA
Lawrance ANBU RAJAN, Yeragudipati RAJALAKSHMI, Vetrivel UMASHANKAR. (2009). Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia coli (Vol. 3). Vol. 3. Journal of Applied Biological Sciences.
MLA
KOPYALA
Lawrance ANBU RAJAN, Yeragudipati RAJALAKSHMI, Vetrivel UMASHANKAR. Molecular Characterization and Sequence Analysis of Trehalose Biosynthesis Genes in Escherichia Coli. no. 55–59, Journal of Applied Biological Sciences, 2009.
Atıf Görüntüle
PDF Görüntüle
Kaynak Görüntüle
Paylaş
Dergi Bilgileri