Journal of Applied Biological Sciences

Journal of Applied Biological Sciences

One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960

Yazarlar: Aytac KOCABAS, Didem SUTAY KOCABAS, Ufuk BAKIR BOLUKBASI

Cilt 5 , Sayı 2 , 2011 , Sayfalar 61-65

Konular:-

Anahtar Kelimeler:Aspergillus terreus,Xylanase,Hydrophobic interaction chromatography,Enzyme characterization

Özet: The aim of study was to investigate purification and characterization of xylanase produced by industrially important strain of Aspergillus terreus. The xylanase was purified by one-step hydrophobic interaction chromatography technique 19-fold with 61% yield. Molecular weight and isoelectric point of the enzyme were determined as 19 kDa and pH 9.0, respectively. The enzyme was found to be unglycosylated. Kinetic experiments at 50°C and pH 7.0 resulted in apparent Km and Vmax values of 2.5±0.05 mg xylan/ml and 50.2±0.4 IU/μg protein, respectively. According to its biochemical properties, the enzyme was found to be a member of family-11 xylanase group. Due to its low molecular weight, the enzyme could be advantageous for industrial applications.

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@article{2011, title={One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960}, volume={5}, number={61–65}, publisher={Journal of Applied Biological Sciences}, author={Aytac KOCABAS, Didem SUTAY KOCABAS, Ufuk BAKIR BOLUKBASI}, year={2011} }
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Aytac KOCABAS, Didem SUTAY KOCABAS, Ufuk BAKIR BOLUKBASI. (2011). One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus terreus NRRL 1960 (Vol. 5). Vol. 5. Journal of Applied Biological Sciences.
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Aytac KOCABAS, Didem SUTAY KOCABAS, Ufuk BAKIR BOLUKBASI. One-Step Purification and Characterization of a Low Molecular Weight Xylanase from Aspergillus Terreus NRRL 1960. no. 61–65, Journal of Applied Biological Sciences, 2011.
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