Journal of Applied Biological Sciences

Yazarlar: Gülden ÇETİN ÖZKAN, Khalid Mahmood KHAWAR

Konular:-

Anahtar Kelimeler:In vitro,Mass propagation,Vicia faba,Rooting,Submerging

Özet: A reliable shoot regeneration system for faba bean (Vicia faba L.) has not been reported to date to build a strong base for genetic transformation in broad bean. The present work reports a rapid, reproducible and proficient regeneration system for faba bean utilizing plumule and embryonic axis explants on MS medium containing 0.25 mg/l BAP +0.50, 0.25, 0.10, 0.05, 0.00 mg / l NAA following submerging of the seeds in distilled water for 24 hours. The explants showed a fast regeneration in 15 days time. These shoots were rooted on MS medium containing 1 mg/l IAA (15 d) and acclimatised on soil peat moss (10 days). The plantlet regeneration reported in this study accounts for effective micropropagation of faba bean. This protocol establishes a strong foundation and fundamental for future genetic transformation studies in faba beans under in vitro conditions

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