European Endodontic Journal

Yazarlar: Ronan Jacques Rezende Delgado, Claudia Ramos Pinheiro, Thaís Helena Gasparoto, Carla Renata Sipert, Ivaldo Gomes De Moraes, Roberto Brandão Garcia, Clóvis Monteiro Bramante, Norberti Bernardineli, Celso Kenji Nishiyama, João Santana Da Silva, Sérgio Aparecido Torres, Gustavo Pompermaier Garlet, Ana Paula Canell

Konular:-

DOI:10.14744/eej.2018.46330

Anahtar Kelimeler: Chronic periapical periodontitis,Cytokines,Lymphocytes,PD-1,PDL-1

Özet: This study aimed to examine programmed death protein 1 (PD-1) and programmed death ligand 1 (PD-L1) expression on leukocytes from chronic apical periodontitis, and to determine the levels of cytokines in the apical periodontitis lesions. Methods: Leukocytes from healthy gingival tissue (n=16) and chronic apical periodontitis (n=10) were evaluated using flow cytometry. The PD-1 and PDL-1 expressions were evaluated using flow cytometry. The cytokine levels were evaluated by enzyme-linked immunosorbent assay. Data were analyzed using one-way ANOVA. The statistical significance level was set at P<0.05. Results: Results showed that the apical periodontitis lesions are more infiltrated by PD-1+ and PDL1+ lymphocytes than the control samples. In addition, the PDL-1 expression was detected on macrophages in the apical periodontitis lesions, and was significantly higher compared to leukocytes from healthy gingival tissue. The IFN-γ, TGF-β, IL-10, and TNF-α levels were significantly higher in the apical periodontitis lesions compared to control samples. Conclusion: The PD-1, PD-L1, and CTLA-4 molecules are evident in apical periodontitis, and can be an important immune checkpoint in chronic periapical periodontitis.

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