International Journal of Veterinary and Animal Research

Yazarlar: Özkan Duru, Hilal Karagül

Konular:

Anahtar Kelimeler:Seminal plasma proteins,SDS page electrophoresis,Sperma,Frozen sperma,Bull

Özet: In this study, seminal plasma proteins were seperated by sodium doedosil sulphate- polyacrylamide gel electrophoresis (SDS-PAGE) and the relationship between obtained protein bands and motility, abnormal number of spermatozoa, abnormal number of dead spermatozoa and spermatozoa density indicating the quality of spermatozoa were examined before and after frozen samples of sperma. Ejaculate samples from 18 holstein bulls were taken and transported to the laboratory in cold conditions. After the examinations of the sperma samples, some of the samples were diluted and frozen. Some other sperma samples were centrifuged to obtain seminal plasma. Proteins of seminal plasma and frozen sperma samples were seperated by SDS-PAGE. Protein bands on the polyacrylamide gel from both seminal plasma and frozen sperma were evaluated as percentage distribution of proteins using densitometer. The percentage distribution of protein bands were compared with statistically the parameters of sperma. Results of this study showed that the protein band with 22 kDa has a relation with the motility and the number of dead spermatozoa. Moreover, the protein band with 29 kDa has a statistically significant relation with the number of abnormal spermatozoa. These results may suggest that seminal plasma proteins have a role on the functions of spermatozoa and its quality of life and on the physiologic role of seminal plasma.

---