Türkiye Entomoloji Dergisi

Yazarlar: Adem ÖZARSLANDAN, Mustafa İMREN, Atilla ÖCAL, İ. Halil ELEKCİOĞLU

Konular:-

Anahtar Kelimeler:Potato,Root-knot nematode,Meloidogyne chitwoodi,Molecular identification

Özet: The root knot nematode (Meloidogyne chitwoodi Golden, O’Bannon, Santo et Finley, 1980) is an important pest of potatoes throughout the world. It is in international quarantine list of many countries. This species was first reported in Niğde and Nevşehir in 2009 Turkey. The objective of the study was to identify the species of root-knot nematodes collected from potato fields in the province of Bitlis where potatoes are grown extensively in 2010. 16 potato tubers were found infected with the root knot nematode. Egg masses and females of root knot nematodes were obtained using binocular stereo microscope from the infected tubers. For the morphological identification of individuals, female vulva and second term juveniles were prepared. At the same time DNA was extracted from egg masses of nematodes and SCAR primers (JMV1, JMV2 and JMVhapla) were used in PCR. Specific SCAR fragment were obtained from the extracted DNA. The multiplex PCR reaction produced the 540 bp fragment for M. chitwoodi. Both the morphological and molecular methods showed that the samples were M. chitwoodi. This study was the first to show the existence of the species in Eastern Anatolian region. The species attacks potato tubers and make them unmarketable due to deformation, lowers the quality of potato chips and french fries for the processing industry.

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