Türkiye Entomoloji Dergisi

Türkiye Entomoloji Dergisi

Insecticidal activities of wild type and recombinant invertebrate iridescent viruses on five common pests

Yazarlar: Donus GENCER, Aydin YESİLYURT, Mustafa GÜLLÜ, İsmail DEMİR, Remziye NALCACIOGLU

Cilt 44 , Sayı 3 , 2020 , Sayfalar 365 - 373

Konular:Fen

DOI:10.16970/entoted.700189

Anahtar Kelimeler:Invertebrate iridescent virus,Insecticidal activity,Recombinant virus

Özet: Invertebrate iridescent virus 6 (IIV6) can infect a broad range of pest insect species. Viruses with new features created by recombinant DNA technology can be used effectively as biological control agents. Previously, recombinants have been constructed: IIVs harboring green fluorescent protein gene (gfp) in place of IIV6 157L ORF (rCIV-Δ157L-gfp) and a scorpion Androctonus australis (Linnaeus, 1758) insect toxin gene (AaIT) fused with gfp (rCIV-Δ157L/gfp-AaIT). In this study, wild type IIV6 and the two recombinants, were evaluated for their ability to cause infections on Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae), Spodoptera littoralis (Boisduval, 1883) (Lepidoptera: Noctuidae), Lymantria dispar (Linnaeus, 1758) (Lepidoptera: Erebidae), Euproctis chrysorrhoea (Linnaeus, 1758) (Lepidoptera: Erebidae) and Tenebrio molitor (Linnaeus, 1758) (Coleoptera: ‎Tenebrionidae) larvae. This study was performed at Karadeniz Technical University, Department of Biology during 2018 and 2019. Five different concentrations (103, 104, 105, 106 and 107 TCID50/ml) of viruses were used to inject each insect larvae. All larvae, infected with rCIV-Δ157L/gfp-AaIT, became paralyzed, except S. littoralis. The LC50 of insect larvae infected by rCIV-Δ157L/gfp-AaIT were determined as 0.3 x 107, 0.7 x 105, 0.2 x 105, 0.15 x 105, 0.7 x 104 TCID50/ml on S. littoralis, T. molitor, L. dispar, H. armigera and E. chrysorrhoea, respectively. LT50 values, calculated according to the highest virus concentrations, were found as 10.5, 6.2, 4.7, 7.5 and 5 d on S. littoralis, T. molitor, L. dispar, H. armigera and E. chrysorrhoea, respectively, for rCIV-Δ157L/gfp-AaIT. This study showed that recombinant IIV6 has increased pathogenicity on some insects from Lepidoptera and Coleoptera.

ATIFLAR
Atıf Yapan Eserler
Henüz Atıf Yapılmamıştır
KAYNAK GÖSTER
BibTex
KOPYALA
@article{2020, title={Insecticidal activities of wild type and recombinant invertebrate iridescent viruses on five common pests}, volume={44}, number={365–373}, publisher={Türkiye Entomoloji Dergisi}, author={Donus GENCER,Aydin YESİLYURT,Mustafa GÜLLÜ,İsmail DEMİR,Remziye NALCACIOGLU}, year={2020} }
APA
KOPYALA
Donus GENCER,Aydin YESİLYURT,Mustafa GÜLLÜ,İsmail DEMİR,Remziye NALCACIOGLU. (2020). Insecticidal activities of wild type and recombinant invertebrate iridescent viruses on five common pests (Vol. 44). Vol. 44. Türkiye Entomoloji Dergisi.
MLA
KOPYALA
Donus GENCER,Aydin YESİLYURT,Mustafa GÜLLÜ,İsmail DEMİR,Remziye NALCACIOGLU. Insecticidal Activities of Wild Type and Recombinant Invertebrate Iridescent Viruses on Five Common Pests. no. 365–373, Türkiye Entomoloji Dergisi, 2020.
Atıf Görüntüle
PDF Görüntüle
Kaynak Görüntüle
Paylaş
Dergi Bilgileri