Journal of Pharmaceutical Technology

Yazarlar: ["Deniz ONAN", "Tilbe ÇEVİKELLİ", "Umay Merve GÜVEN", "Esra DEMİRTÜRK"]

Konular:-

DOI:10.37662/jpt.2022.1000275

Anahtar Kelimeler:Quercetin,Microemulsion based gel,Validation,HPLC

Özet: Abstract This study validated a high performance liquid chromatography (HPLC) method for the determination of quercetin (QE) in microemulsion (ME) based gel formulations. The analyses were performed on a C18 column (150x4.6 mm, 5 m particle size) at room temperature with UV detection at 254 nm. The mobile phase was composed of methanol:water (65:35, v/v, 2% acetic acid) mixture, and flow rate was set to 1 mL/min. The method was validated according to the international guidelines with respect to linearity range, stability, limit of quantitation and detection, precision and accuracy. The method was linear within 5 – 100 µg/mL range with a correlation coefficient of 0.9995. The intra- and inter-assay precisions presented RSD values lower than 2%. The method reported is a fast and reliable HPLC method useful for QE determination in ME based gel formulations.

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