Harran Üniversitesi Tıp Fakültesi Dergisi

Yazarlar: Lokman VARISLI, Abdullah OZGONUL, Osman CEN

Konular:-

Anahtar Kelimeler:VDR,Bioinformatics,Comparative genomics,In silico Biology

Özet: Background: The aims of this study was to analyze some properties of this gene and its products; such as the homologous protein sequences to human VDR molecule, the common transcription factor binding sites on promoter sequences, phylogenetic relationships of the conserved domains, and in silico expression profiles based on EST data that presence in ESTdb in all investigated. Methods: We investigated the homology, conserved domain, promoter and expression profiles of the VDR gene based on EST data from the ESTdb by using bioinformatics tools in different species. Results: The results of this study indicated that VDR molecule is middling conserved among all studied organisms; for example, human similar to Macaca mulatta 98% and Saguinus oedipus 98% , to Petromyzon marinus 59% . From the point of view of ZnF_C4 C4 zinc finger in nuclear hormone receptors and HOLI Ligand binding domain of hormones domains, it was obtained several similar motifs. Separately, it was seen that there was significantly difference gene expression in normal and cancer tissues of brain in human. Conclusion: This study demonstrated that there was no common transcription factor binding sites in promoter VDR gene in different species. Phylogenetic trees constructed using the neighbor-joining method NJ revealed a close evolutionary relationship of ZnF_C4 and HOLI in various species

---