Harran Üniversitesi Tıp Fakültesi Dergisi

Yazarlar: Fuat DILMEC, Ali MATUR, Emre ALHAN, Soner UZUN, Mehmet KARAKAS, Derya ALABAZ, Nurten AKSOY, Feridun AKKAFA, Necmi AKSARAY, Hamdi R

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Anahtar Kelimeler:Identification,Leishmaniasis,PCR,RFLP.

Özet: Background: Leishmaniasis, caused by parasites of the genus Leishmania is still considered an important health problem. We aimed to appraise a Polymerase Chain Reaction-Restriction Fragment Length Polymorphism PCR-RFLP method for the detection and species differentiation of Leishmania parasites in clinical samples; and to compare with the results of microscopic examination. Methods: Totally 58 samples were taken from 45 skin lesion, nine bone marrow aspirates, and four reference strains. The samples were used for both DNA and smear-slide preparations. The DNAs were amplified by PCR for the detection of Leishmania subgenus and PCR products were restricted with HaeIII for the species differentiation. Results: The visceral Leishmania parasites VL were genotyped as Leishmania infantum. Of 45 patients with CL, most of them were analyzed as Leishmania tropica, but the others were characterized similar to Leishmania donovani reference. It was also determined positivity rate in PCR 100% was higher than microscopic examination about 67% . Conclusions: PCR-RFLP technique appears to be most sensitive for the detection and the differentiation of Leishmania species

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