Harran Üniversitesi Tıp Fakültesi Dergisi

Yazarlar: Çiğdem GÜNGÖRMEZ, Hatice Gumushan AKTAS, Ersin BORAZAN

Konular:-

Anahtar Kelimeler:MiRNA,Oncogenic miRNA,Colorectal cancer,Biomarker

Özet: Background: In this study, we aimed to detect the expression levels of miR-92, miR-21 and miR-155 in tumor and normal colon and rectum tissues with oncogenic microRNA (miRNA) obtained from patients with Stage II colorectal cancer using qRTPCR and to compare the obtained data. Material and Method: This study was carried out with the clinical, laboratory and pathological findings of the tissues obtained from patients diagnosed with colorectal cancer at Gaziantep University Medical Faculty Research and Training Hospital -General Surgery Department. miRNeasy mini kit-isolated miRNAs were used for qRT-PCR implementation. The isolated miRNAs were converted into cDNA via the miScript RT-PCR commercial kit. The cDNA samples with miRNA expression levels in tissue samples were identified via Rotor Gene RT-PCR system using RT-SYBR Green qPCR kit. Result: The mean level of miRNA-21 expression in 9 patients diagnosed with colorectal cancer appears to have increased 2.09 fold compared to normal tissue and tumor tissue, while miR-155 increased 2.22 fold. This increase was considered as statistically significant (p <0.05). When the fold chance values of normal and tumor tissue are compared, despite being oncogenic miRNA, miR-92 appears to have decreased 1.37 fold and was not considered statistically significant (p>0.05). Conclusion: According to the findings obtained in our study, the expression levels of oncogenic miRNA miR21 and miR-155 in tumor colon tissues of patients with colorectal cancer have increased. The results of recent studies have shown that miRNAs involved in the regulation of many cellular functions may be used as biomarkers for diagnosis and treatment of cancer.

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